COMPARATIVE STUDY OF SOME OXIDOREDUCTASES’ IMMOBILIZATION ON FILMS, MICRO- AND NANOPARTICLE SUPPORTS
E. NEAGU, G. PAUN, V. MOROEANU, V. PARVULESCU, G. L. RADU

Abstract. A comparative study of laccase and peroxidase (oxidoreductases) immobilization on polymeric supports and silica nanoparticles (SBA15 and SBA15 NH₂) is presented. Two types of biocompatible polymeric supports were obtained: films of sodium alginate/chitosan/hyaluronic acid, simple and with magnetic nanoparticles, and microparticles of chitosan and/or sodium alginate (as polymers) ± hyaluronic acid. Such supports were used to immobilize laccase and peroxidase by two methods: direct immersion and after support activation with 2.5% glutaraldehyde. For comparison, some supports based on silica, simple or functionalized (SBA15 and SBA15 NH₂) also were used to immobilize above mentioned enzymes. The immobilization yields of peroxidase on microparticles were calculated, the highest ones being obtained in case of supports activated with glutaraldehyde: 98,89% for chitosan microparticles and 98,86% for those
of alginate + chitosan. In case of peroxidase immobilized on silica supports, simple and functionalized (SBA15 and SBA15 NH₂), the immobilization yields were lower against that obtained using organic supports, namely, 98% and respectively 96,26%. As regards the laccase immobilization, the obtained immobilization yields using chitosan microparticles were 99,8%, while using silica supports they were above 90,8% and 90,7%. The properties of the used polymers and their structure determine the possibility to use them as raw materials in obtaining biocompatible supports in order to immobilize some biological active substances (enzymes, drugs).